Platelet crossmatching using the SPRCA technique is widely used to select platelet products for patients who have become refractory. The use of crossmatched platelets may improve transfusion outcome for these individuals on an interim basis until HLA-matched products are available or as continuous transfusion support when the transfusion outcome is favorable. Platelets crossmatched against maternal serum can also be used to support neonates in some cases of NAIT, depending on antibody specificity.
Platelet crossmatch testing detects IgG antibodies to platelet-specific and HLA antigens. A serum or plasma sample from the patient is tested against apheresis platelets. Depending on the antibody, compatible platelets may or may not be readily available. The crossmatching program, in partnership with the HLA matching service, provides transfusion support and medical consultation for refractory patients who are difficult to support by standard methods.
- Glycoprotein-specific ELISA
For human platelet antigen (HPA) typing by molecular methods, visit our Molecular Testing page.