HLA Class I antibodies are also detected in GAT and GIFT so the monoclonal antibody immobilization of neutrophil antigens (MAINA) test is used to differentiate HNA antibodies from HLA antibodies. In the MAINA test, HLA Class I antibodies are removed, leaving only the HNA antibodies available for detection.
We can utilize the neutrophil crossmatch to aid in the diagnosis of alloimmune neonatal neutropenia and TRALI. We also test for drug dependent neutrophil antibodies. Both of these tests are available upon request.
When HNA antibody has been identified it is important to confirm the HNA antibody specificity by determining the patient's or donor's neutrophil antigen type. Both serological (phenotype) and molecular (genotype) methods are used to determine the neutrophil antigen type. The following neutrophil associated antigens are detected by each method.
- HNA-1a/1b/1c, HNA-3a/3b, HNA-4a/4b, HNA-5a/5bw
- HNA-1a/1b/1c, HNA-2, HNA-3a, HNA-4a