Find a Blood Drive
Find a Blood Drive
Home

Human Leukocyte Antigen Testing

Human Leukocyte Antigen (HLA) Testing

Human Leukocyte Antigen (HLA) Testing

American Red Cross Blood Services is dedicated to providing the safest, most cost-effective blood products and services. Our HLA laboratories provide comprehensive and state-of-the-art HLA services supporting hematopoietic cell transplantation (HCT), Solid Organ Transplantation, Transfusion, Disease/Drug Association Study, Vaccine research, and more.

HLA laboratories also provide DNA based typing for genetic polymorphisms of cytokine genes, minor histocompatibility antigens and natural killer cell immunoglobulin-like receptor (KIR) genes, and for post-HCT engraftment monitoring using short tandem repeat (STR) markers.

Contact Information

Services available at HLA laboratories may vary, so please contact your Red Cross representative to discuss your needs.

If you are not yet able to order HLA testing services through Connect, please complete and submit the form for your regional HLA laboratory using the appropriate form: 

  • Detection and identification of HLA antibodies in sensitized patients and donors
  • Determination of HLA types of patients and blood donors to assist in the provision of HLA-matched platelet transfusions
  • HLA testing to support HCT and organ transplantation, including monitoring of engraftment post-HCT transplantation
  • Identification of disease predisposition HLA gene for Celiac disease, narcolepsy, Ankylosing spondylitis and others
  • Identification of drug hypersensitivity related HLA genes, including: –HLA-B*5701 with Abacavir –HLA-B*1502 with Carbamazepine –HLA-B*5801 with Allopurinol and others

Red Cross HLA laboratories are CLIA-certified and accredited by agencies such as the American Society for Histocompatibility and Immunogenetics (ASHI), the United Network of Organ Sharing, the National Marrow Donor Program (NMDP), the New York Department of Health, and the Florida Health Agency. HLA services include:

  • HLA Typing
    • HLA Genes: HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1 and -DPB1
    • Resolution: Low, Intermediate, High and Allelic
    • Throughput: Low, High
  • HLA Antibody Analysis
  • T and B Cell Crossmatching
  • Disease and Drug Sensitivity Association Study
  • Platelet Antibody Screening
  • Platelet Crossmatching
  • HLA Matched Platelet for Transfusion
  • Chimerism/Engraftment Monitoring
    • Whole Blood
    • Whole Marrow
    • CD3, CD4 and CD8 T Cell
    • CD19/20 B Cell
    • CD33/66 Myeloid Cell
    • CD56 NK Cell
    • CD71
  • KIR Typing
  • Relationship (Paternity) testing
  • Identical Twin Verification
  • CCR5 Delta 32 Mutation Test
  • Additional services available
  • HLA typing: Next Generation Sequencing (NGS), Sanger SBT, PCR-SSP, PCR-Sequence Specific Oligonucleotide Probeis (SSOP)
  • HLA antibody detection/identification: Luminex Multi-Antigen Bead, Luminex Single Antigen Bead (SAB), AHG-CDC, or ELISA methods
  • Engraftment monitoring: STR-PCR
  • Disease/Drug sensitivity association: NGS, Sanger SBT, PCR-SSP, PCR-SSOP
  • KIR Typing: Sanger SBT, PCR-SSP, PCR-SSOP
  • Platelet Alloimmunization Screen/Crossmatch: Solid Phase Red Cell Adherence Assay (SPRCA)
  • T and B Cell Crossmatching: CDC, AHG-CDC