Human Leukocyte Antigen Testing

Human Leukocyte Antigen (HLA) Testing

American Red Cross Blood Services is dedicated to providing the safest, most cost-effective blood products and services. Our HLA laboratories provide comprehensive and state-of-the-art HLA services supporting hematopoietic cell transplantation (HCT), Solid Organ Transplantation, Transfusion, Disease/Drug Association Study, Vaccine research, and more.

HLA laboratories also provide DNA based typing for genetic polymorphisms of cytokine genes, minor histocompatibility antigens and natural killer cell immunoglobulin-like receptor (KIR) genes, and for post-HCT engraftment monitoring using short tandem repeat (STR) markers.

Contact Information

Services available at HLA laboratories may vary, so please contact your Red Cross representative to discuss your needs.

Charlotte 704-347-8205
Dedham 781-461-2148
Portland 503-528-5754
Philadelphia 215-451-4131
Pomona 909-859-7415

If you are not yet able to order HLA testing services through Connect, please complete and submit the form for your regional HLA laboratory using the appropriate form:

Indications

Detection and identification of HLA antibodies in sensitized patients and donors
Determination of HLA types of patients and blood donors to assist in the provision of HLA-matched platelet transfusions
HLA testing to support HCT and organ transplantation, including monitoring of engraftment post-HCT transplantation
Identification of disease predisposition HLA gene for Celiac disease, narcolepsy, Ankylosing spondylitis and others
Identification of drug hypersensitivity related HLA genes, including: –HLA-B*5701 with Abacavir –HLA-B*1502 with Carbamazepine –HLA-B*5801 with Allopurinol and others

Description

Red Cross HLA laboratories are CLIA-certified and accredited by agencies such as the American Society for Histocompatibility and Immunogenetics (ASHI), the United Network of Organ Sharing, the National Marrow Donor Program (NMDP), the New York Department of Health, and the Florida Health Agency. HLA services include:

HLA Typing
- HLA Genes: HLA-A, -B, -C, -DRB1, -DRB3, -DRB4, -DRB5, -DQA1, -DQB1, -DPA1 and -DPB1
- Resolution: Low, Intermediate, High and Allelic
- Throughput: Low, High
HLA Antibody Analysis
T and B Cell Crossmatching
Disease and Drug Sensitivity Association Study
Platelet Antibody Screening
Platelet Crossmatching
HLA Matched Platelet for Transfusion
Chimerism/Engraftment Monitoring
- Whole Blood
- Whole Marrow
- CD3, CD4 and CD8 T Cell
- CD19/20 B Cell
- CD33/66 Myeloid Cell
- CD56 NK Cell
- CD71
KIR Typing
Relationship (Paternity) testing
Identical Twin Verification
CCR5 Delta 32 Mutation Test
Additional services available

Test Methods

HLA typing: Next Generation Sequencing (NGS), Sanger SBT, PCR-SSP, PCR-Sequence Specific Oligonucleotide Probeis (SSOP)
HLA antibody detection/identification: Luminex Multi-Antigen Bead, Luminex Single Antigen Bead (SAB), AHG-CDC, or ELISA methods
Engraftment monitoring: STR-PCR
Disease/Drug sensitivity association: NGS, Sanger SBT, PCR-SSP, PCR-SSOP
KIR Typing: Sanger SBT, PCR-SSP, PCR-SSOP
Platelet Alloimmunization Screen/Crossmatch: Solid Phase Red Cell Adherence Assay (SPRCA)
T and B Cell Crossmatching: CDC, AHG-CDC